| 摘要 |
<FORM:1097173/C2/1> An antifugal antibiotic, pyrrolnitrin, 3-(2-nitro - 3 - chlorophenyl) - 4 - chloro - pyrrole, is prepared by cultivating a pyrrolnitrin producing strain of Pseudomonas genus in a liquid culture medium under aerobic conditions until substantial amount of the antibiotic is produced. Species of Pseudomonas specified as Ps. pyrrocinia (Nov. Spec.), Ps. pyrrolnitrica (Nov. Spec.), Ps. aeruginosa, Ps. mephitica, Ps. ovalis and Ps. schuylkilliensis. Pyrrolnitrin is recovered from the fermentation broth by treating the centrifuged cell cake with a solvent, e.g. acetone, removing impurities from the extract by treatment with active carbon, concentrating the extract and re-extracting into another solvent for the antibiotic, e.g. butyl acetate, washing the extract with aqueous acid and/or alkali, concentrating and precipitating by means of a non-solvent, e.g. cyclohexane. Further purification may be by (a) chromatography on alumina, magnesium silicate or silica gel and (b) recrystallization from mixed solvent, e.g. methanol - cyclohexane. Pyrrolnitrin is soluble in many solvents, e.g. methanol, acetone, ether, petroleum, benzine, benzene and butyl acetate, but is sparingly soluble in water and cyclohexane. I.R. (Fig. 1), U.V. (Fig. 2, not shown). Fig. 3 (not shown) is the curve of the nuclear magnetic resonance spectrum. Pharmaceutical preparation for topical application, e.g. alcoholic solutions and ointments, comprise pyrrolnitrin alone or with other medicaments for the treatment of fungal infections.
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