| 摘要 |
PURPOSE:To obtain the subject gene capable of mass-producing a 5-substituted hydantoin racemase converting DL-5-substituted hydantoin into the corresponding optically active amino acid by coding the aforementioned enzyme, integrating the coded enzyme in a vector and making a host hold the vector. CONSTITUTION:A plasmid of a microorganism NS671 (FERM P-9543) belonging to the genus Pseudomonas is treated with a restriction enzyme, linked to a vector and transduced into Escherichia coli to carry out transformation. Screening is then performed to select a clone containing a DNA capable of coding a 5-substituted hydantoin racemase, and a plasmid is collected from the resultant clone and treated with a restriction enzyme to provide a 5-substituted hydantoin racemase gene having a sequence from the 202nd base to the 951st base expressed by the formula. Furthermore, the resultant gene is linked to a vector and transduced into Escherichia coli to preform transformation. The obtained transformant is cultured in a culture medium to afford the objective 5-substituted hydantoin racemase that is a protein, composed of 249 amino acids and having 27090 molecular weight. |
