摘要 |
The invention relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention also relates to a composition comprising (i) an enzyme with nucleic acid polymerase activity, (ii) an inert protein and, (ii) a zwitterionic detergent. The invention further relates to a method for enzymatic nucleic acid synthesis comprising the steps of, (a) providing in a reaction mixture, a polymerase activity, a nucleic acid template, a zwitterionic detergent, a buffer, a salt, nucleotides and an inert protein and, (b) incubating the reaction mixture at a temperature which enables nucleic acid synthesis. |
申请人 |
QIAGEN GMBH;FANG, NAN;LOEFFERT, DIRK;ERBACH, CHRISTOPH;PETERS, LARS-ERIK |
发明人 |
FANG, NAN;LOEFFERT, DIRK;ERBACH, CHRISTOPH;PETERS, LARS-ERIK |