ANIMAL MODEL PROTOCOL, DIAGNOSTIC, THERAPEUTIC AND VACCINE AGAINST DIGITAL DERMATITIS

摘要

Applicants have discovered a novel induction method for inducing digital dermatitis lesions consistent with the natural disease process. Applicants have prepared a consortium including a macerated tissue culture that may be used in the method as well as a novel inoculation procedure. The consortia of microbes includes isolates of Treponema phagedenis, Porphorymonas, and Baceteroides and the invention includes use of these novel isolates in preventing and treating digital dermatitis. The invention can be used to screen, test or compare the relative efficacy of various drugs and biologics for use in treatment or control of digital dermatitis.

主权项

1. A method of generating an animal model of digital dermatitis comprising:
abrading a foot of an animal subject; wrapping said foot, inoculating the abraded foot with a composition comprising a culture of macerated digital dermatitis tissue, and thereafter removing the wraps, wherein said treatment results in formation of digital dermatitis lesions in the interdigital space, and does not include a water maceration step.