发明名称 |
L-amino acid producing bacterium |
摘要 |
A method for producing an L-amino acid is described, for example, L-phenylalanine and L-histidine, by fermentation using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified by attaching a DNA fragment able to be transcribed encoding the peptide represented in SEQ ID NO: 2, or a variant thereof, particularly a portion of the ssrA gene, to the 3′-end of gene encoding for the bacterial enzyme, which influences on the L-amino acid biosynthesis, such as chorismate mutase/prephenate dehydrogenase or phosphoglucose isomerase. |
申请公布号 |
US9376695(B2) |
申请公布日期 |
2016.06.28 |
申请号 |
US200812238704 |
申请日期 |
2008.09.26 |
申请人 |
AJINOMOTO CO., INC. |
发明人 |
Shakulov Rustem Saidovich;Klyachko Elena Vitalievna;Doroshenko Vera Georgievna;Airikh Larisa Gotlibovna |
分类号 |
C12P13/22;C12P13/20;C12P13/24;C12P21/02;C12N9/02;C12N9/90;C12N9/92 |
主分类号 |
C12P13/22 |
代理机构 |
Cermak Nakajima & McGowan LLP |
代理人 |
Cermak Shelly Guest;Cermak Nakajima & McGowan LLP |
主权项 |
1. A recombinant L-amino acid producing Escherichia coli bacterium having a DNA comprising:
A) a gene selected from the group consisting of tyrA, pheA, pgi, ilvE, ilvA, tdcB, sdaA, sdaB, argA, argG, proB, thrB, and combinations thereof, and B) a DNA fragment able to be transcribed and encoding the peptide of SEQ ID NO: 2, or a variant thereof consisting of a deletion, insertion, substitution, or addition of 1 amino acid as compared to SEQ ID NO: 2, wherein said DNA fragment of B) is attached to the 3′ end of said gene of A) and consequently enhances production of an L-amino acid. |
地址 |
Tokyo JP |