发明名称 |
Droplet-based particle sorting |
摘要 |
The present invention relates to droplet-based particle sorting. According to one embodiment, a method of providing a droplet comprising a single cell is provided, wherein the method includes providing a droplet comprising a suspension of cells on an electrowetting droplet actuator surrounded by an oil medium, dispensing from the droplet to provide a dispensed droplet, determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single cell, and sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations. |
申请公布号 |
US9395329(B2) |
申请公布日期 |
2016.07.19 |
申请号 |
US201514805532 |
申请日期 |
2015.07.22 |
申请人 |
ADVANCED LIQUID LOGIC, INC. |
发明人 |
Pamula Vamsee K.;Pollack Michael G.;Srinivasan Vijay |
分类号 |
G01N1/18;G01N35/10;G01N27/447;G01N15/00 |
主分类号 |
G01N1/18 |
代理机构 |
Ward and Smith, P.A. |
代理人 |
Ward and Smith, P.A. ;Simmons Ryan K.;Fazzolare David A. |
主权项 |
1. A method of providing a droplet comprising a single cell, the method comprising:
(a) providing a droplet comprising a suspension of cells on an electrowetting droplet actuator surrounded by an oil medium; (b) dispensing from the droplet of (a) to provide a dispensed droplet, wherein the dispensing is electrowetting-mediated; (c) determining whether the dispensed droplet on the electrowetting droplet actuator comprises a single cell; and (d) sorting the dispensed droplet on the electrowetting droplet actuator based on the results of the determining step using electrowetting-mediated droplet operations. |
地址 |
San Diego CA US |