| 摘要 |
1,224,768. Purification of antibiotic 833A. MERCK & CO. Inc. 12 Sept., 1968 [22 Jan., 1968], No. 43437/68. Heading C2A. Antibiotic 833A, i.e. ( - )(cis-1,2-epoxypropyl) phosphonic acid, obtained according to Specification 1,223,924 is purified by passing a fermentation broth or a solution containing said antibiotic through a column of an anion exchange resin or a cation exchange resin, eluting the resin adsorbate with an eluting solvent, collecting the eluate, and combining the active fractions. The anion exchange resin may be composed of quaternary ammonium exchange groups attached to a styrene divinyl benzene polymer lattice. The cation exchange resin may be composed of nuclear sulphonic acid exchange groups attached to a styrene divinylbenzene polymer lattice. The eluting solvent may be an aqueous alcoholic solution of a water-soluble alkali metal, NH 4 or tris-(hydroxymethyl) aminomethane salt. Further purification is obtained by concentrating the combined active fractions, dissolving the residue in water, chromatographing the resulting aqueous solution on polyacrylamide gel of 50 to 200 mesh capable of operating on substances of molecular weight from 200-2000. |
