Method for the isolation of nucleic acids

摘要

The present invention concerns an improved method for the isolation of nucleic acids such as DNA and RNA from bacterial, plant, animal or human cells as well as from cell cultures and virus cultures, wherein the nucleic acid is immobilised on a matrix having a silicon-oxygen compound in the presence of a chaotropic agent and an alkanol, carried out in a temperature range of 36° to 75° C.

主权项

1. In a method for the isolation and/or purification of a nucleic acid comprising:
a) lysis of a biological sample, b) immobilization of a released nucleic acid on a matrix having one or more silicon-oxygen compounds in the presence of a chaotropic compound and a branched or linear alkanol, (c) separation of the bound nucleic acid from said sample, and wherein and optionally washing the nucleic acid immobilized on the matrix following step (b) the improvement comprises immobilization of the nucleic acid at a temperature range of 46° C. to 75° C.