Heterologous expression of fungal cellobiohydrolase 2 genes in yeast

摘要

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms Cochliobolus heterostrophus, Gibberella zeae, Irpex lacteus, Volvariella volvacea, and Piromyces sp. in host cells, such as the yeast Saccharomyces cerevisiae. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

主权项

1. An isolated polynucleotide comprising a nucleic acid which encodes a cellobiohydrolase or a functional fragment thereof, wherein at least 70% of the codons of said nucleic acid is codon-optimized for expression in a heterologous yeast host cell and wherein the cellobiohydrolase has an amino acid sequence of SEQ ID NO: 11, SEQ ID NO: 12, SEQ ID NO: 13, SEQ ID NO: 14, or SEQ ID NO: 15.