Plasmide und Wirtszellen zur Expression von INA Protein (Ice nucleating protein).
摘要
Additives for promoting ice nucleation in ingestible biological materials at sub-zero temperatures comprising a protein capable of promoting said ice nucleation carried by GRAS microorganisms transformed by a vector carrying one or more genes coding for the expression of said protein or by fractions of said microorganisms. Said vector is a plasmid comprising in the correct reading frame a cloning vector and DNA coding for said protein capable of promoting ice-nucleation and in the case of a yeast GRAS microorganism substantially the complete yeast 2 micron plasmid, at least one yeast selectable auxotrophic marker and the dLEU2 allele of the LEU2 gene and, in the case of a Lactococcal GRAS microorganism, additionally a non-inducible erythromycin resistance gene and a lactococcal plasmid promoter and origin of replication.
申请公布号
DE69013458(T2)
申请公布日期
1995.02.23
申请号
DE1990613458T
申请日期
1990.10.15
申请人
SOCIETE DES PRODUITS NESTLE S.A., VEVEY, CH
发明人
HOTTINGER, HERBERT, CH-1807 BLONAY, CH;NIEDERBERGER, PETER, CH-1066 EPALINGES, CH;PRIDMORE, DAVID, CH-1009 PULLY, CH;STAEGER-ROOSE, URSULA, CHEONG JU CITY 360-112, KR