| 摘要 |
<p>PURPOSE:To obtain human T cell hybridoma producing human B cell growth factor or human B cell differentiation factor and capable of being cultured permanently. CONSTITUTION:The hypoxanthine-guanine-phosphoribosyl-transferase-less human leukemia T cell derived from human leukemia T cell is used as a parent cell, and is fused with human T cell separated and purified from various T cells derived from peripheral blood, marrow, lymph node, spleen, etc., in a proper medium in the presence of a fusion accelerating agent (e.g. a polyetylene glycol having an average molecular weight of 1,000-6,000). The fused cells are cultured in a conventional medium for selection of hybrid, e.g. a medium containing hypoxanthine, aminopterin and thymidine to separate the desired fused cell. The obtained human T cell hybridoma strain is proliferated in a conventional medium to cause cloning, and is separated to the separate fused cell strains.</p> |
