METHOD OF PREPARING DIMERIC SINGLE-CHAIN FUSED PROTEIN (VARIANTS)

摘要

FIELD: biotechnology, chemistry of proteins. SUBSTANCE: DNA sequences of the first and the second monomeric units encoding a single-chain Fv-fragment of antibody and amphiphilic spiral peptide are cloned in one of two expressing vector. Host cells are transformed with an expression vector (amu) and cultured and the end product expressed in cells or medium is isolated. Method ensures to obtain dimeric fused proteins of high activity against the corresponding antigens. Invention can be used for making fused proteins of antibody fragment exhibiting bi- or multifunctional binding sites. EFFECT: improved method of preparing. 3 cl, 5 dwg, 8 ex