摘要 |
The method entails the binding protein being degraded by a thiol which cleaves SH groups in the alkaline range and is characterised in that a 1,2-dithiolane-3-carboxylic acid of the formula (I) <IMAGE> in which n is an integer from 1 to 8, is used as thiol which cleaves SH groups, and the method is used to prepare samples for analytical and investigative methods for determining the liberated analyte, in particular for immunoassays for vitamin B12. |