| 摘要 |
The invention is directed towards a method of amplifying target nucleic acid s by using two oligonucleotide probe complement pairs. Each member of the probe pair contains a chemical functionality group which permits linkage of the probes when the functionality groups are adjacent to one another following hybridization of the probe pairs to th e template. One probe in each pair is composed of two regions, a first region which hybridizes to the target and which contains the chemica l functionality group and a second, protecting region which prevents target independent joining as shown in the figure. The other probe in the pair contains the corresponding chemical functionality group. Upon joining of a first probe pair, an amplification can proceed in which the newly joined first probe pair can serve as a template for the second, complementary probe pair, which can in turn serve as a template for unjoined first probe pairs. This cyclic amplification is sensitive enough for a discriminative amplification of sequences which diffe r by merely a point mutation and therefore is suitable for point mutation detection and genotype determination as well as for the determinati on of the presence or absence of a specific nucleic acid in a sample.
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