STRAIN OF BACTERIUM PAENIBACILLUS CAMPINASENSIS AS PRODUCER OF CYCLODEXTRIN GLUCANOTRANSFERASE

摘要

FIELD: biotechnology, microbiology, biochemistry. ^ SUBSTANCE: invention relates to a novel strain of microorganisms producing cyclodextrin glucanotransferase (CGTase; EC 2.4.1.19) with the enhanced specificity with respect to formation of beta-cyclodextrin. The strain of bacterium Paenibacillus campinasensis IB-10155 is deposited and stored in Collection of microorganisms in Biology Institute UNTS RAN. Activity of CGTase in cultural fluid is estimated to be 5.8 U/ml wherein 1 unit of activity is determined as amount of CGTase catalyzing formation of 1 mcM of ACB beta-cyclodextrin per 1 minute from 2% starch solution at pH = 6.0. Part of beta-cyclodextrin in sum of other cyclic products exceeds 70%. Invention provides high stability of CGTase catalyzing the transformation reaction of starch to beta-cyclodextrin at relative constant part of this product to sum of other cyclic dextrins in early steps of reaction. ^ EFFECT: valuable properties of strain. ^ 2 tbl, 4 ex