RECOMBINANT TYPE II COLLAGENASE FROM CLOSTRIDIUM HISTOLYTICUM AND ITS USE FOR ISOLATING CELLS AND GROUPS OF CELLS

摘要

Process for disintegrating cell tissue and releasing cells or groups of cells contained therein by incubation of the cell tissue with a class II collagenase from Clostridium histolyticum, which still has a considerable degree of collagenase activity after 12 freeze/thaw cycles and contains SEQ ID NO: 2 or SEQ ID NO:10 in a characteristic partial region or it is coded in this region by SEQ ID NO: 1, SEQ ID NO: 9 or a sequence which codes for the same amino acid sequence within the scope of the degeneracy of the genetic code and is the product of a prokaryotic or eukaryotic expression of an exogenous DNA, until the cells or groups of cells are released to the desired extent and separating the cells or the groups of cells from the cell tissue fractions.