METHOD OF AND COMPOSITE FOR FLUORESCENT IN SITU HYBRIDIZATION
摘要
A fluorescent in situ hybridization method including the steps of (a) obtaining a chromosome spread of a species; (b) preparing a hybridization composite containing a plurality of chromosomal paints each of the plurality of chromosomal paints being labeled with a different fluorophore-or-combination-of-fluorophores, such that an averaged specific activity of highly repetitive sequences in the hybridization composite substantially equals an averaged specific activity of unique sequences in the hybridization composite; (c) denaturing the hybridization composite and subjecting the hybridization composite to conditions for allowing at least a part of the highly repetitive sequences in the hybridization composite to reanneal while at least a part of the unique sequences in the hybridization composite remaining single stranded; (d) contacting under hybridization conditions the hybridization composite with the chromosome spread; (e) washing away excess of the hybridization composite; and (f) analyzing and presenting images of the now hybridized chromosome spread.
申请公布号
WO0006773(A1)
申请公布日期
2000.02.10
申请号
WO1999US16626
申请日期
1999.07.23
申请人
APPLIED SPECTRAL IMAGING;BAR-AM, IRIT;JANISH, CATHY;GARINI, YUVAL;CABIB, DARIO;BUCKWALD, ROBERT, A.
发明人
BAR-AM, IRIT;JANISH, CATHY;GARINI, YUVAL;CABIB, DARIO;BUCKWALD, ROBERT, A.