发明名称 |
DIRECT GENE TRANSFER INTO THE RUMINANT MAMMARY GLAND |
摘要 |
Disclosed is a method for expressing a heterologous gene in the mammary gland of a ruminant mammal. The invention is useful for producing a heterologous protein(s) in the milk of the mammal, for producing a heterologous enzyme(s) which is tethered to the mammary epithelial cell membrane and which can act on a component of the milk of the mammal, and for providing an antisense oligonucleotide(s) or ribozyme(s) which inhibits expression of a gene to a mammary epithelial cell. The method involves infusing a liquid complex including a genetic construct into a ductal tree of the mammal. The liquid complex can be infused before the mammal reaches sexual maturity and after the mammal develops a functional streak canal. Alternatively, a liquid complex that is free of live retroviruses can be infused. If desired, the infused genetic construct can be treated with a polycationic compound and/or a lipid to improve the efficiency with which it is taken up by an epithelial cell of the mammary gland. |
申请公布号 |
WO9622379(A2) |
申请公布日期 |
1996.07.25 |
申请号 |
WO1996CA00029 |
申请日期 |
1996.01.19 |
申请人 |
NEXIA BIOTECHNOLOGIES, INC. |
发明人 |
KARATZAS, CONSTANTINOS;LAZARIS-KARATZAS, ANTHOULA;TURNER, JEFFREY, DONALD |
分类号 |
A61K38/00;C07K14/61;C12N9/38;C12N9/72;C12N15/85 |
主分类号 |
A61K38/00 |
代理机构 |
|
代理人 |
|
主权项 |
|
地址 |
|