STRAIN A/47/TEXAS/91/2/6(H1N1) FOR PRODUCTION OF LIVING INTRANASAL INFLUENZA VACCINE FOR CHILDREN

摘要

FIELD: virology, medicine. SUBSTANCE: vaccine strain A/47/Texas/91/2/6 - reassortant obtained by crossing epidemic virus A/Texas/36/91 (H1N1) with cold-adopted temperature-sensitive virus A/Leningrad/134/47/57 (H2N2) - attenuation donor. The latter is harmless for children and adult and can be used for preparing reassortant vaccine strain from newly appearing epidemic viruses. Strain A/47/Texas/91/2/6 is actively propagated in chicken embryos at optimal temperature 32-34 C, shows temperature-sensitivity and cold-adaptation. Reassortant inherited two genes from epidemic virus encoding surface proteins (hemagglutinin and neuraminidase) and six genes from attenuation donor encoding nonglycosylated proteins. Strain A/47/Texas/91/2/6 (H1N1) is areactogenic for children of age 3-15 years at intranasal administration. EFFECT: preparing vaccine strain that corresponds actual virus A/Texas/36/91 (H1N1).