Method for culturing mammalian cells to improve recombinant protein production

摘要

The present invention relates to methods for mammalian cell culture. The methods make use of independent tyrosine and cystine feed streams.

主权项

1. A method of culturing Chinese Hamster Ovary (CHO) cells expressing a recombinant protein, comprising growing the CHO cells in a defined serum-free culture medium during a growth phase and maintaining the CHO cells in the cell culture medium during a production phase by supplementing the cell culture with a concentrated serum-free defined feed medium containing tyrosine and further supplementing the cell culture with one or more independent tyrosine feeds, wherein the independent tyrosine feed provides at least about 1 mM to at least about 2 mM tyrosine at each feed, wherein viability was prolonged, specific productivity was maintained, and titer was improved compared to CHO cells not receiving independent tyrosine feeds.