COMBINATORIAL BIOSYNTHESIS OF 5-O-DESOSAMINYL ERYTHRONOLIDE A AS A PRECURSOR OF KETOLIDE ANTIBIOTICS AND DEVELOPMENT OF ITS RECOMBINANT STRAIN

摘要

A combination biosynthetic method of 5-O-desosaminyl erythronolide A which is a precursor Ketolides antibiotic is provided to reduce a process for removing L-cladinose adhered to the C-3 position of erythromycin A. A combination biosynthetic method of 5-O-desosaminyl erythronolide A comprises the steps of: (i) producing a glycosyltransferase gene eryBV adhering an L-mycarose ugar chain by acting on Saccharopolyspora erythraea erythronolide B; and (ii) cultivating a strain which additionally generates a substrate-thixotropic cytochrome P450 monooxygenase heterologous gene pikC in an Streptomyces venezuelae-derived pikromycin biosynthetic pathway, to Saccharopolyspora erythraea eryBV-deletion mutant and extracting the 5-O-desosaminyl erythronolide A from the culture fluid.