Codierende DNS für ein Enzym, das die Position- 4'' von makrolidischen Antibiotika azyliert

摘要

A DNA fragment containing acyB gene coding for an enzyme capable of acylating the 4 sec -position of a macrolide antibiotic, said DNA fragment being derived from a microorganism of the genus Streptomyces, having a size of about 3.1 kb and being characterized by the restriction endonuclease map shown in Figure 1, and a DNA restriction fragment resulting from digestion with a restriction endonuclease; a recombinant DNA plasmid containing this DNA fragment; a microorganism transformed with this recombinant plasmid; and a process for producing a 4 sec -acylated macrolide antibiotic by using the transformant.