发明名称 |
Culture system and method for maintenance and proliferation of undifferentiated human embryonic stem cells |
摘要 |
Provided herein are human embryonic stem cells and their method of culturing. In one embodiment, the human embryonic stem cells are maintained in an environment containing an extracellular matrix isolated from feeder cells and a conditioned medium pre-conditioned by the feeder cells; the feeder cells are pre-inactivated by either gamma ray radiation or by mitomycin C treatment. The cultured human embryonic stem cells remain substantially undifferentiated and maintain their pluripotency to differentiate into three germ layer cells. |
申请公布号 |
AU2004237789(A1) |
申请公布日期 |
2005.06.23 |
申请号 |
AU20040237789 |
申请日期 |
2004.12.07 |
申请人 |
INDUSTRIAL TECHNOLOGY RESEARCH INSTITUTE |
发明人 |
MEI-JU YANG;CHAO-YING KUO;WANN-HSIN CHEN;JUN-JAE HUANG;LIH-TAO HSU;KUANG-NING CHANG |
分类号 |
C12M3/00;C12N5/00;C12N5/02;C12N5/0735;(IPC1-7):C12N5/08 |
主分类号 |
C12M3/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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