发明名称 TDCBC/PCKA GENE-INACTIVATED MICROORGANISM AND METHOD OF PRODUCING L-THREONINE USING THE SAME
摘要 The present invention provide a microorganism comprising an inactivated chromosomal tdcBC gene and an inactivated chromosomal pckA gene, which has remarkably improved productivity of L-threonine. Also, the present invention provides a method of producing L-threonine using the microorganism. The microorganism is prepared by incorporating by a recombination technique an antibiotic resistance gene into a pckA gene on the chromosome of a bacterial strain containing an L-threonine degradation-associated operon gene, tdcBC, which is inactivated. The microorganism has the effect of preventing degradation and intracellular influx of L-threonine due to the inactivation of the tdcBC operon gene, and includes more activated pathways for L-threonine biosynthesis. Therefore, the microorganism is useful for mass production of L-threonine because of being capable of producing L-threonine in high levels and high yields even in the presence of high concentrations of glucose.
申请公布号 HU0501100(A3) 申请公布日期 2010.01.28
申请号 HU20050001100 申请日期 2004.04.02
申请人 CJ CHEILJEDANG CORPORATION 发明人
分类号 C12N1/21;C12N9/88;C12P13/08 主分类号 C12N1/21
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