Indicator Molecules For Use In Detecting Enzyme Cleavage Activity

摘要

The present invention relates to indicator molecules containing multiple cleavage sites for use in detecting enzyme cleavage activity. The invention also relates to various applications of these indicator molecules, for example in enzyme detection devices, particularly although not exclusively, devices for use in the detection of enzyme activity in a test sample. The invention also relates to using the indicator molecules in methods for detecting the presence of enzyme activity in a test sample.

主权项

1. An indicator molecule for use in the detection of enzyme cleavage activity in a test sample comprising:
(i) an enzyme modifiable region comprising multiple separate cleavage sites; and (ii) a fluorophore, wherein cleavage at one or more of the multiple separate cleavage sites causes a measurable change in fluorescence of the fluorophore.