METHOD FOR EXPRESSING AND SECRETING KERATINASE

摘要

The present invention provides a Bacillus subtilis host cell capable of expressing and secreting keratinase. The host cell contains a recombinant DNA molecule comprising vector DNA and DNA encoding Bacillus licheniformis PWD-1 keratinase enzyme operatively associated therewith. The present invention also provides a method for producing keratinase enzyme. The method includes the steps of (a) culturing a Bacillus subtilis host cell containing a recombinant DNA molecule comprising vector DNA and DNA encoding Bacillus licheniformis PWD-1 keratinase enzyme operatively associated therewith, and (b) collecting keratinase enzyme from the cell culture.