A SELF-ASSEMBLED MONOLAYER FOR UREASE IMMOBILIZATION OF BIOSENSOR

摘要

A self-assembled monolayer for urease immobilization of a biosensor is provided to enhance sensitivity at low urea concentration, reduce reaction time of the biosensor by direct electron transfer of the self-assembled monolayer, and especially enhance sensitivity of the biosensor when porous silicone is used as a substrate. The self-assembled monolayer for urease immobilization of the biosensor is used as an immobilization membrane of the biosensor and is manufactured with 3-mercaptopropionic acid, wherein the biosensor is prepared by depositing the gold thin layer on the surface of a planar electrode and porous silicone to produce an electrode, and forming the self-assembled monolayer on the gold thin layer. The urea sensor comprises the self-assembled monolayer made of 3-mercaptopropionic acid as the immobilization membrane.