| 摘要 |
PURPOSE:To enable the separation and analyzation of components at a high accuracy by adding very small amount of hydroxy propyl methylcellulose to an electrolytic solution for a tubular type uniform speed electrophoretic analysis leading liquid as thickener to inhibit an electric osmotic current even in an alkaline area. CONSTITUTION:A leading liquid containing the ion of the same character with higher mobility than an ion to be analyzed is arranged to ajoin a terminal liquid containing the ion of the same character with lower mobility than the ion being analyzed on the interface in a capillary tube and a sample is injected into the interface while a constant current flows from both ends of the tube to separate and analyze the ion being analyzed in the ascending order of mobility. In this invention, 0.01-0.2% of hydroxy propyl methylcellulose is added to the leading liquid to thicken. This can inhibit the electric osmotic current even in the not only acid to neutral but also alkaline area to prevent components separated from running over a slip to the capillary tube making the boundary between the components unclear thereby enabling separation and analysis at a high accuracy. |
