发明名称 |
MUTANT PROTEINASE WITH REDUCED SELF-CLEAVAGE ACTIVITY AND METHOD OF PURIFICATION |
摘要 |
The present invention provides a mutant 27 kDa NIa proteinase having reduced self-cleavage activity relative to the self-cleavage activity of its wild-type proteinase. The mutant has the same substrate cleavage activity as the wild-type proteinase but is more stable than the wild-type proteinase. The present invention also provides a method of obtaining large quantities of active 27 kDa NIa proteinase for use as a tool for purification of other proteins. |
申请公布号 |
US2008318273(A1) |
申请公布日期 |
2008.12.25 |
申请号 |
US20080184315 |
申请日期 |
2008.08.01 |
申请人 |
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发明人 |
DOUDNA JENNIFER A.;LUCAST LOUISE J.;BATEY ROBERT T. |
分类号 |
C12P21/02;C07H21/04;C12N1/21;C12N9/50;C12N15/63 |
主分类号 |
C12P21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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