发明名称 |
Purification of microbial cell fermentation homogenates |
摘要 |
A method for purifying a desired heterologous polypeptide from microbial fermentation broth or homogenate in which it is produced and solubilized is described. This method involves adding to the broth or homogenate an effective amount of a solution of 6,9-diamino-2-ethoxyacridine lactate (ethacridine lactate) to precipitate host cell impurities under conditions wherein the majority of polypeptide remains soluble, and separating the desired polypeptide from the broth or homogenate. The broth or homogenate containing the ethacridine lactate and polypeptide is also disclosed.
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申请公布号 |
US7579448(B2) |
申请公布日期 |
2009.08.25 |
申请号 |
US20060609529 |
申请日期 |
2006.12.12 |
申请人 |
GENENTECH, INC. |
发明人 |
LESTER PHILIP M.;PERSSON JOSEFINE |
分类号 |
C12P21/02;A61K39/395;C07D219/00;C07H21/04;C07K16/18;C12N1/18;C12N1/20 |
主分类号 |
C12P21/02 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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