摘要 |
Methods and materials are disclosed for the inhibition and control of gibberellic acid levels. In particular, nucleic acid sequences of copalyl diphosphate synthase, 3- beta hydroxylase, and 2-oxidase and additional nucleic acid sequences are disclosed. Gibberellic acid levels may be inhibited or controlled by preparation of a chimeric expression construct capable of expressing a RNA or protein product which suppresses the gibberellin biosynthetic pathway sequence, diverts substrates from the pathway or degrades pathway substrates or products. The sequence is preferably a copalyl diphosphate synthase sequence, a 3 beta -hydroxylase sequence, a 2-oxidase sequence, a phytoene synthase sequence, a C20-oxidase sequence, and a 2 beta ,3 beta -hydroxylase sequence. Administration of a complementing agent, preferably a gibberellin or gibberellin precursor or intermediate restores bioactivity.
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申请人 |
MONSANTO COMPANY;BROWN, SHERRI, M.;ELICH, TEDD, D.;HECK, GREGORY, R.;KISHORE, GANESH, M.;LOGUSCH, EUGENE, W.;LOGUSCH, SHERRY, J.;PILLER, KENNETH, J.;RAO, SUDABATHULA;REAM, JOEL, E. |
发明人 |
BROWN, SHERRI, M.;ELICH, TEDD, D.;HECK, GREGORY, R.;KISHORE, GANESH, M.;LOGUSCH, EUGENE, W.;LOGUSCH, SHERRY, J.;PILLER, KENNETH, J.;RAO, SUDABATHULA;REAM, JOEL, E. |