| 摘要 |
<p>1,176,968. Determining amino acid arylamidase. ARZNEIMITTELWERK DRESDEN VEB. 8 Sept., 1967, No. 41197/67. Heading B1X. A composition for the determination of amino acid arylamidase (leucinaminopeptidase) comprises (a) alanine hydrazide, alanine pnitroanilide or alanine-#-naphthylamide, (b) a buffer solution with a pH of 6.5 to 7.5, with or without (c) an aromatic aldehyde colour reagent, for example p-dimethylamino-benzaldehyde. In use, the sample, for example blood serum, is incubated with the substrate and the buffer solution. When alanine p-nitroanilide is used, the p-nitro-aniline formed is determined colorimetrically, optionally in a kinetic test. When alanine hydrazide or alanine-#-naphthylamide is used, the hydrazine or #-naphthylamine formed react with the aromatic aldehyde colour reagent and the solution is acidified to give a coloured salt, the extinction of which is measured. A test pack contains solutions of the alanine derivative and of the buffer, optionally mixed together, with or without a solution of the colour reagent.</p> |
