发明名称 |
Transformed human pluripotent stem cells and associated methods |
摘要 |
The present disclosure provides transformed human pluripotent stem cell (t-hPSC). t-hPSCs are not dependent on Oct4 for renewal and survival, however exhibit a sensitivity to reduced levels of the transcription factor Nanog. Also provided are methods of culturing cells for use in a cell-based screening assay comprising placing one or more transformed human pluripotent stem cells into a receptacle and culturing said stem cells in the receptacle to form a monolayer of stem cells without cell overlap. Methods of screening compounds using t-hPSCs are also described. |
申请公布号 |
US9365821(B2) |
申请公布日期 |
2016.06.14 |
申请号 |
US201414329412 |
申请日期 |
2014.07.11 |
申请人 |
MCMASTER UNIVERSITY |
发明人 |
Bhatia Mickie;Werbowetski-Ogilvie Tamra;Sachlos Eleftherios;Fischer Russell Daniela;Laronde Sarah;Lee JungBok;Szabo Eva;Risueno Ruth |
分类号 |
C12N15/00;C12N5/0735;G01N33/50;C12N5/095 |
主分类号 |
C12N15/00 |
代理机构 |
Ferris H. Lander, Inc. |
代理人 |
Ferris H. Lander, Inc. |
主权项 |
1. A transformed human pluripotent stem cell (t-hPSC) produced by the variant human embryonic stem cell line deposited with the International Depositary of Canada (IDAC), under Accession No. 220715-01, and designated v-hESC-1, wherein the cell does not require bFGF for maintenance of an undifferentiated state, maintains expression of SSEA3 in the absence of bFGF, co-expresses FGFR1 and IGFR1, has an amplification of at least 0.8 megabase at 20q 11.1-11.2 and grows in a monolayer. |
地址 |
Hamilton, Ontario CA |