Recombinant polypeptide construct comprising multiple enterotoxigenic Escherichia coli fimbrial subunits

摘要

The inventive subject matter relates to a recombinant polypeptide constructs comprising enterotoxigenic Escherichia coli fimbrial subunits. The recombinant polypeptide constructs comprise multiple subunits to the same or different ETEC fimbrial types. The constructs are useful for inclusion in immunogenic formulations for the induction of immunity against entertoxigenic Escherichia coli. The inventive subject matter also relates to the use of the recombinant polypeptide constructs in induce anti-enterotoxigenic Escherichia coli immunity.

主权项

1. A method of inducing an immune response in a mammal comprising the steps:
a. administrating one or more priming dose a recombinant polypeptide construct, as a subunit vaccine or expressed in a suitable expression vector, in a buffered aqueous solution, wherein said comprises a recombinant polypeptide construct comprising a whole or immunogenic fragment of an Escherichia coli fimbrial minor or major subunit connected to one or more major fimbrial subunits or immunogenic fragments, thereof, of the same fimbrial type, via a polypeptide linker, and wherein each of the one or more major fimbrial subunits contain a donor β strand and are also connected to each other via a polypeptide linker, wherein the C-terminal Escherichia coli fimbrial major subunit is connected, via a linker, to a C-terminal donor β strand derived from a major Escherichia coli fimbrial subunit that is homologous or heterologous to the immediately N-terminal major subunit and wherein said construct can comprise a histidine tag at the C-terminus; b. administrating one or more boosting doses with first dose at least 1 week after priming dose with a unit dose range of 1 μg to 1 mg of immunogen in a buffered aqueous solution, wherein an immune response is elicited.