| 摘要 |
Described herein are methods and materials useful for improving lateral root growth, water uptake, and the yield of grain of cereal grasses grown under drought stress conditions. In particular, the present disclosure provides a quantitative trait locus associated with improved yield under drought stress. The disclosure further provides recombinant DNA for the generation of transgenic plants, transgenic plant cells, and methods of producing the same. The present disclosure further provides methods for generating transgenic seed that can be used to produce a transgenic plant having improved yield under drought stress, and methods for improving yield under drought stress in a cereal grass involving marker assisted selection and backcrossing. |
| 主权项 |
1. A method of improving lateral root growth and water uptake in a cereal grass comprising:
a) crossing a crossing plant of one variety of cereal grass having chromosomal DNA that comprises a nucleic acid comprising qDTY12.1, or a yield-improving part thereof, with a recipient plant of a distinct variety of cereal grass having chromosomal DNA that does not include a nucleic acid comprising qDTY12.1, or a yield-improving part thereof; and b) selecting one or more progeny plants having chromosomal DNA that comprises a nucleic acid comprising qDTY12.1, or a yield-improving part thereof, wherein qDTY12.1, or a yield-improving part thereof, is detected in the crossing plant, recipient plant, or one or more progeny plants by analyzing genomic DNA from the crossing plant, the recipient plant, or one or more progeny plant, or germplasm, pollen, or seed thereof, for the presence of at least one molecular marker linked to qDTY12.1, or a yield-improving part thereof, wherein the at least one molecular marker is selected from the group consisting of: RM28048; RM28076; RM28089; RM28099; RM28130; RM511; RM1261; RM28166; RM28199; and Indel-8, and wherein a selected one or more progeny plant having DNA that comprises a nucleic acid comprising qDTY12.1, or a yield-improving part thereof, has improved lateral root growth and water uptake. |
