| 主权项 |
1. A method for determining whether a Chikungunya virus (CHIKV) nucleic acid sequence is present in a test sample comprising nucleic acids, said method comprising the steps of:
(a) contacting nucleic acids of the test sample with a set of amplification oligonucleotides,
wherein a first member of said set is up to 100 bases in length and complementary to at least 15 contiguous bases contained within SEQ ID NO:14, andwherein a second member of said set is up to 100 bases in length and complementary to at least 15 contiguous bases of an extension product of the first member of said set of amplification oligonucleotides when a polynucleotide consisting of SEQ ID NO:14 is the template in a template-dependent primer extension reaction; (b) performing an in vitro nucleic acid amplification reaction using nucleic acids of the test sample as templates together with said set of amplification oligonucleotides, whereby, if said test sample comprises said CHIKV nucleic acid sequence, there is produced an amplification product; and (c) detecting any of said amplification product that may have been produced in the in vitro nucleic acid amplification reaction,
wherein detecting said amplification product in an amount greater than a cutoff value indicates that the CHIKV nucleic acid sequence is present in the test sample, andwherein detecting said amplification product in an amount less than the cutoff value indicates that the CHIKV nucleic acid sequence is absent from the test sample. |
