Verbessertes Verfahren zur Bestimmung von Lipase

摘要

The invention concerns a method and a reagent for determining lipase in the presence of a colored substrate and at least one N-substituting carboxamide derivative or a mixture of the general formula (I) or (Ia), in which R<1> and R<2> independently represent hydrogen or a saturated or non-saturated, substituting or non-substituting hydrocarbon residue with 2 to 24 carbon atoms, Z a saturated or non-saturated, substituting, cyclic or linear-chain hydrocarbon residue with 1 to 10 carbon atoms, X an atom or atom group with positive charge and n a figure ranging from 1 to 3. Specially tetranatrium-N-(1,2-dicarboxylethyl)-N-alkyl-sulfo-succinamide or a mixture containing such compound has proven to be quite advantageous in eliminating unspecified reactions during the determination of lipase in a biological test sample.