发明名称 |
Method for isolation of bovine low-molecular weight CR-binding substance and method of use of the same |
摘要 |
A fully chromium loaded bovine low-molecular weight chromium-binding protein is isolated by a process that combines homogenization with supplementation of chromium content. Following homogenization with water, the homogenate is fractionated with ethanol, and the fractions obtained are subjected to serial chromatography (ion-exchange followed by size-exclusion chromatography) to obtain the biologically pure bovine LMWCr. This biologically pure material elutes from an HPLC column as essentially a single band, giving a high degree of purity. The LMWCr is useful as a dietary supplement, and for the treatment or prevention of a variety of chromium-related disease conditions.
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申请公布号 |
US5872102(A) |
申请公布日期 |
1999.02.16 |
申请号 |
US19960729591 |
申请日期 |
1996.10.11 |
申请人 |
THE UNIVERSITY OF ALABAMA |
发明人 |
VINCENT, JOHN B.;DAVIS, C. MICHELE |
分类号 |
C07K14/47;(IPC1-7):C07K19/00 |
主分类号 |
C07K14/47 |
代理机构 |
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代理人 |
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地址 |
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