| 摘要 |
PURPOSE: The FADD-DD mutant proteins and genes encoding for the same are provided, thereby the FADD-DD mutant proteins capable of selectively transferring the cell death signal of Fas or TNF-alpha(tumor necrosis factor-alpha) can be prepared and the proteins can be effectively used in study on chemical therapy and cell death by stress. CONSTITUTION: The FADD-DD(Fas-associated death domain protein-death domain) mutant protein has the amino acid sequence of which 110th amino acid is substituted with an amino acid selected from glycine, alanine, valine, leucine and isoleucine. The FADD-DD mutant protein R110A, wherein 110th amino acid of the FADD-DD protein is substituted with alanine, has the amino acid sequence of SEQ ID NO: 11. A gene encoding for the FADD-DD mutant protein R110A has the nucleotide sequence of SEQ ID NO: 5. The FADD-DD mutant protein has the amino acid sequence of which 117th amino acid is substituted with an amino acid selected from glycine, alanine, valine, leucine and isoleucine. The FADD-DD mutant protein R117A, wherein 117th amino acid of the FADD-DD protein is substituted with alanine, has the amino acid sequence of SEQ ID NO: 12. A gene encoding for the FADD-DD mutant protein R117A has the nucleotide sequence of SEQ ID NO: 7. |
