| 摘要 |
PROBLEM TO BE SOLVED: To provide a photodetection-type electrophoretic apparatus by which a fragment is detected at high speed and with high sensitivity. SOLUTION: In this apparatus, a fragment in which a nucleic acid sample is fluorescence-labeled is being electrophoresed by using a migration medium 4, a separated fragment is irradiated with a laser beam 1, fluorescence which in generated from a fluorescence labeling operation is detected, and the base sequence of the nucleic acid sample is decided. At this time, a polyacrylamine concentration in the migration medium 4 is decided in such a way that the migration time required when a fragment in a required base length is electrophoreses at the prescribe distance of the migration medium 4 becomes minimum, and the polyacryalamide concentration is set to a range of 2 to 6%, thereby shortening the measuring time of a fragment up to a 300 base length to 1.5 hours or lower. |
