| 摘要 |
Provided is an oligonucleotide which shows excellent hybridization specificity to various pathogens causing sexually transmitted diseases and has excellent workability on a multiplex PCR so as to simultaneously detect the various pathogens causing sexually transmitted diseases in one PCR reaction set. The oligonucleotide specifically hybridized into nucleic acid of pathogen causing sexually transmitted diseases is represented by the general formula of 5'-Xp-Yq-Zr-3', wherein Xp is a 5'-high Tm specificity portion having a hybridizing sequence substantially complementary to a target sequence to be hybridized, Yq is a separation portion having at least two universal nucleotides, Zr is a 3'-low Tm specificity portion having a hybridizing sequence substantially complementary to the target sequence to be hybridized, each p, q, and r is a number of nucleotides, each X, Y and Z is a deoxyribonucleotide or a ribonucleotide, the Tm of the 5'-high Tm specificity portion is higher than the Tm of the 3'-low Tm specificity portion, the separation portion has the lowest Tm among the three portions, the separation portion forms a non-base pair bubble structure on the condition that the 5'-high Tm specificity portion and the 3'-low Tm specificity portion are hybridized into the target sequence, the bubble structure allows the 5'-high Tm specificity portion to be separated from the 3'-low Tm specificity portion, the specific separation allows the hybridization specificity of a DSO total structure to be determined by the 5'-high Tm specificity portion and the 3'-low Tm specificity portion, thereby improving the hybridization specificity of the DSO total structure, the pathogen causing sexually transmitted diseases is Mycoplasma hominis, Ureaplasma urealyticum, Neisseria gonorrheae, Chlamydia trachomatis, Herpes simples virus-1 or 2, Candida albicans, Haemophilus ducreyi, Trichomonas vaginalis, Mycoplasma genitalium, Treponema pallidum or Gardnella vaginalis, and the target sequence is a nucleic acid sequence of the pathogen causing sexually transmitted diseases. |
