Methods for analysis of isomeric lipids

摘要

A method for analyzing a sample that contains a plurality of lipid isomers is described that involves forming one or more lipid metal ion adducts and transporting the one or more lipid metal ion adducts through a differential mobility spectrometer to cause separation of the one or more lipid metal ion adducts from each other. The lipid isomers can be chosen, for example, from fatty acids, glycerolipids, glycerophospholipids, sphingolipids, saccharolipids, polyketides, sterol lipids, and prenol lipids. Particular examples include phosphatidylcholine regioisomers such as 1-palmatoyl-2-oleoyl-sn-phosphatidylcholine (POPC) and 1-oleoyl-2-palmatoyl-sn-phosphatidylcholine and triacylglycerols containing palmetic and oleic acid groups. The metal chosen can include a cationization reagent that contains sodium, potassium, silver or lithium.

主权项

1. A method for analyzing a sample, comprising:
a. ionizing a sample containing or suspected of containing a plurality of lipid isomers so as to form one or more lipid silver adducts, at least one of the plurality of lipid isomers being selected from the group consisting of fatty acids, glycerolipids, glycerophospholipids, sphingolipids, saccharolipids, polyketides, sterol lipids, and prenol lipids, and wherein an acyl chain of the at least one of the plurality of lipid isomers comprises at least one unsaturated carbon-carbon double bond; and b. transporting said one or more lipid silver adducts through a differential mobility spectrometer to effect separation of said one or more lipid silver adducts; wherein ionizing the sample comprises reacting the sample with a salt of silver.