Improved vaccines for proliferative ileitis and methods of making and using the same

摘要

A proliferative ileitis vaccine comprising tissue culture grown Lawsonia intracellularis and methods of making said vaccines. Proliferative ileitis vaccines described include those containing whole L. intracellularis, extracts of L. intracellularis, protective immunogenic subunits of L. intracellularis, recombinant immunogens of L. intracellularis and naked DNA of L. intracellularis. The vaccines of this invention may be inactivated or modified live and contain adjuvants and/or stabilizers. The vaccines of this invention may be in a liquid or lyophilized form. Also disclosed are monoclonal antibodies which neutralize the growth of L. intracellularis and which may be used for diagnosing proliferative ileitis as well as for quantitating antigen during vaccine production.