Cell culture processes

摘要

Culturing heterologous protein-secreting mammalian cells, such as CHO or BHK cells, at 35.1-36.5° C. and/or at pH 7.15-7.20 and/or at a dissolved CO2 concentration of 10% or lower. Preferred heterologous proteins are Factor VIII, ADAMTS-13, furin or Factor VII.

主权项

1. A method comprising:
expressing heterologous Factor VIII protein in a mammalian cell in continuous cell culture wherein the continuous cell culture is maintained at a temperature of 36.0±0.5° C. and a cell culture density of from 1.4×106 to 2.6×106 cells/ml, and wherein the mammalian cell is a CHO cell.