| 摘要 |
<p>To prepare hetero-bispecific monoclonal antibodies, at least the genes for the light chain and for the variable part of the heavy chain are isolated from a hybridoma cell line which secretes an antibody with a required specificity, and they are inserted into a eukaryotic plasmid vector which contains a selectable marker, together with a strong promoter, this expression vector is transfected into a hybridoma cell line which secretes antibodies with a second required specificity, the cell line is cultured, the antibodies are isolated, and the bispecific antibody is removed. <IMAGE></p> |
