Efficient method for establishing induced pluripotent stem cells

摘要

The invention provides a method of improving the efficiency of establishment of induced pluripotent stem cells by increasing, in a nuclear reprogramming step of somatic cell, the level of activated form of one or more proteins selected from the group consisting of Ras family members, PI3 kinase, RalGEF, Raf, AKT family members, Rheb, TCL1 and S6K. The invention also provides a method of producing induced pluripotent stem cells by contacting a somatic cell with a nuclear reprogramming substance and one or more of such proteins and nucleic acids that encode such proteins. The invention further provides an induced pluripotent stem cell that has an exogenous nucleic acid encoding such a protein, as well as agents for use in the aforesaid methods.

主权项

1. A method of producing induced pluripotent stem cells, comprising contacting a somatic cell with a nuclear reprogramming substance(s) and a nucleic acid vector(s) encoding one or more AKT family members, thereby producing functionally pluripotent stem cells,
wherein the nuclear reprogramming substance(s) comprises a nucleic acid vector encoding Oct3/4 and optionally comprises a nucleic acid vector(s) encoding one or more substances selected from the group consisting of Sox2, Klf4, c-Myc, Lin28 and Nanog.