Polyfocal Interferometric Image Acquisition

摘要

A microscope-based system and method for simultaneous imaging of several object planes, of a three-dimensional (3D) sample, associated with different depths throughout the sample. The system includes a polyfocal optical portion, adapted to create a plurality of optical channels each of which is associated with an image of a corresponding object plane, and a spectrally-selective portion, adapted to transform the spectral distribution of the image-forming beam of light to a corresponding spatial distribution. The image, registered by a detector, includes an image of an object plane and an image of the spatially-coded spectral distribution. The method effectuates the simultaneous multispectral imaging of the several object planes. The required data-acquisition time is several fold shorter than that taken by a conventional multispectral microscope-based imaging system.

主权项

1. A method for imaging a sample with an optical system having an input, the method comprising:
receiving, along an optical axis of the input, light associated with said sample and having a first spectral distribution; spatially redirecting the received light into optical channels, each optical channel having a corresponding effective optical power; and detecting light that has transmitted through each of the optical channels with a photodetector such as to fill an aperture of the photodetector with a plurality of substantially non-overlapping images, of said sample, respectively corresponding to optical channels, wherein each optical channel is configured to transmit light having a first spectral distribution, and wherein different optical channels have different effective optical powers.