| 摘要 |
<p>PROBLEM TO BE SOLVED: To provide a method for efficient cultivation of liver cells, in which liver cells collected from a matured mammal are cultured in a medium containing pleiotrophin, fetal bovine serum, an ascorbic acid and nicotinamide, to form the colony. SOLUTION: This method for efficient successive cultivation of liver cells comprises (A) primary cultivation to form the colony, in which liver cells collected from a matured mammal are cultured in a DMEM medium or the like containing pleiotrophin, fetal bovine serum, an ascorbic acid, nicotinamide, DMSO and epidermal growth factor, (B) separation of the colony cells from the medium by the aid of an EDTA/trypsin solution, (C) dispersion of the separated liver cells individually, and (D) cultivation of these individually dispersed liver cells in a medium containing pleiotrophin, fetal bovine serum, an ascorbic acid, nicotinamide, DMSO and epidermal growth factor, to form the colony. The liver cell is useful as a research material for studying, e.g. its evolution, differentiation, division, canceration mechanisms involved or the like, or as a medical material, e.g. for treatment of liver diseases.</p> |
