COLUMN AND METHOD FOR PREPARING A BIOLOGICAL SAMPLE FOR PROTEIN PROFILING

摘要

The present invention relates to a liquid chromatography column having a solid support comprising at least one immobilised crown ether. It also relates to a use of a crown ether for preparing a biological sample for protein profiling. The invention also relates to a method of preparing a biological sample for protein profiling, comprising the steps of: treating (2) the sample (1) with a cleavage reagent to generate peptides (7) comprising N-terminal primary amines (3, 4, 5), and sorting (9, 10) the peptides (7) by non-covalent interactions using a solid support (11, 13), wherein the non-covalent interactions are H-bridges or pi-pi (p-p) interactions. It also relates to a kit therefor.