Multi-functional option valve, multifunctional fully automatic liquid chromatography system including the same, and method for analyzing sample using the same

摘要

Provided is a multifunction selection valve for use in a liquid chromatography system. The multifunction selection valve includes multiple ports to which both ends of a 1st dimension separation column may be connected at a part thereof, the multifunction selection valve including: a fluid passing mode in which a fluid introduced thereto is not passed through the 1st dimension separation column but is discharged; a column passing mode in which the fluid introduced thereto is passed through the 1st dimension separation column and then discharged; and a fluid blocking mode in which the fluid is prevented from being introduced. When using the multifunction selection valve, it modifies the flow paths of chromatographic solvents so that the resultant multifunctional liquid chromatography system allows one-dimensional separation, two-dimensional separation, on-line digestion and on-line phosphopeptide enrichment by a single binary pump.

主权项

1. A multifunction selection apparatus having multiple ports to which both ends of a 1st dimension separation column are connected at a part thereof,
the multiple ports comprising an inlet port, an outlet port, and a first connecting port and a second connecting port linked individually to each end of the 1st dimension separation column the multifunction selection apparatus further comprising: a fluid passing mode in which a fluid introduced thereto is not passed through the 1st dimension separation column but is discharged, the inlet port is directly and fluidically communicated with the outlet port in the fluid passing mode; a column passing mode in which the fluid introduced thereto is passed through the 1st dimension separation column and then discharged, the inlet port is fluidically communicated with the first connecting port of the 1st dimension separation column and the outlet port is fluidically communicated with the second connecting port of the 1st dimension separation column in the column passing mode; and a fluid blocking mode in which the fluid is prevented from being introduced, the inlet port and the outlet port are fluidically interrupted with each other in the fluid blocking mode, wherein the multiple ports further comprise a first, a second and a third selection ports; the second selection port is fluidically communicated with the third selection port and the second connecting port of the 1st dimension separation column is fluidically communicated with the first selection port, in the fluid passing mode; the second selection port is fluidically communicated with the third selection port, in the column passing mode; and the first connecting port of the 1st dimension separation column is fluidically communicated with the second selection port, the second connecting port of the 1st dimension separation column is fluidically communicated with the third selection port, and the first selection port is fluidically communicated with the outlet port, in the fluid blocking mode.